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A New Method for Express Detection of Antibiotic Resistance
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Journal Article
A New Method for Express Detection of Antibiotic Resistance
2023
Overview
The oscillation mode of an atomic force microscope (AFM) was used to create a highly sensitive real-time detection system for antibiotic resistance. This mode allows one to evaluate the sensitivity or resistance of Gram-negative (
Escherichia coli
) and Gram-positive (
Staphylococcus aureus
) bacteria to an antibiotic in 15–30 minutes. The analytical signal (changes in the amplitude-frequency characteristics of the cantilever) is based on the metabolic activity of bacteria. Bacteria were placed on the cantilever and causing it to oscillate with high amplitude. If the bacteria are sensitive to the antibiotic, the amplitude drops statistically significant within 15–30 minutes, if the bacteria are resistant, then the amplitude either does not change or increases. The results were comparable with the disk diffusion method.
Publisher
Pleiades Publishing,Springer Nature B.V
Subject
