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The Root-Tuber Trypsin Inhibitor of Winged Bean and Its Anti-cancerous Activity Against Osteosarcoma Cell-Line
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The Root-Tuber Trypsin Inhibitor of Winged Bean and Its Anti-cancerous Activity Against Osteosarcoma Cell-Line
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Journal Article
The Root-Tuber Trypsin Inhibitor of Winged Bean and Its Anti-cancerous Activity Against Osteosarcoma Cell-Line
2025
Overview
Trypsin inhibitor from the root-tuber of underutilized legume Winged bean (Psophocarpus tetragonolobus (L.) DC.) (WbT-TI) was purified using ion exchange chromatography followed by size-exclusion chromatography. The purified WbT-TI showed a molecular mass of 20,609 Da and an isoelectric point of 5.10. Ultraviolet circular dichroism (UV-CD) and intrinsic fluorescence reported, that WbT-TI interacts with trypsin. Domain-wise analysis of WbT-TI revealed it to belong to the Kunitz-type soybean trypsin inhibitor (STI) family with a specific β-trefoil fold. The sequence of WbT-TI showed 44% sequence coverage to acidic trypsin inhibitor from the seed of the same plant. Protein interaction similarity analysis (PIPSA) evaluated the electrostatic properties of WbT-TI and provided information about the interacting partners of trypsin inhibitors. The purified protein was quantified and tested for in vitro anticancer activity using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay against the human osteosarcoma (MG-63) cell line. At 5 µg/ml of WbT-TI, the highest inhibition was seen. These studies may lead to the development of winged bean protease inhibitor-based preventive and therapeutic strategies for different kinds of cancers.
Publisher
Springer,Springer Nature B.V
Subject
/ Antineoplastic Agents, Phytogenic - chemistry
/ Antineoplastic Agents, Phytogenic - isolation & purification
/ Antineoplastic Agents, Phytogenic - pharmacology
/ Ethylenediaminetetraacetic acid
/ Humans
/ Legumes
/ Plant Proteins - isolation & purification
/ Plant Proteins - pharmacology
/ Proteins
/ Sarcoma
/ Size exclusion chromatography
/ Soybean
/ Soybeans
/ Trypsin
/ Trypsin Inhibitor, Kunitz Soybean - chemistry
/ Trypsin Inhibitor, Kunitz Soybean - isolation & purification
/ Trypsin Inhibitor, Kunitz Soybean - pharmacology
/ Trypsin Inhibitors - chemistry
/ Trypsin Inhibitors - isolation & purification
