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An improved and reliable molecular sexing technique for Asiatic black bears, Ursus thibetanus
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An improved and reliable molecular sexing technique for Asiatic black bears, Ursus thibetanus
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Journal Article
An improved and reliable molecular sexing technique for Asiatic black bears, Ursus thibetanus
2013
Overview
We developed an improved and reliable molecular genetics technique for sexing Asiatic black bear,
Ursus thibetanus
. Individual sex determination based on differential banding patterns of
SRY
and
Amelogenin
genes were taken advantageously to perform a duplex PCR and sexes were correctly assigned to known and unknown individuals using capillary electrophoresis. Molecular sexing based on fluorescent labeled primers was also found to be appropriate for non-invasive samples, and raised the applicability of multiplexing with microsatellites.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
