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Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli
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Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli
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Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli
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Journal Article
Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli
2024
Overview
Abstract
The increasing trend of carbapenem resistance amongst Escherichia coli poses a major public health crisis and requires active surveillance of resistance mechanisms to control the threat. Quorum-sensing system plays a role in bacterial resistance to antibiotics. Quorum sensing is a cell–cell communication system where bacteria alter their gene expression in response to specific stimuli. Here, in this study we investigated the transcriptional response of quorum-sensing receptor, sdiA in E. coli under sub-inhibitory concentration of carbapenem in the presence of quorum-sensing signal molecules. Two E. coli isolates harbouring blaNDM were subjected to treatment with 10% Sodium Dodecyl Sulphate (SDS) for 20 consecutive days of which blaNDM encoding plasmid was successfully eliminated from one isolate. Both the wild type and the cured mutant were then allowed to grow under eight different inducing conditions and the transcriptional response of sdiA gene was studied by quantitative real-time Polymerase Chain Reaction (PCR) methodt. We found different response levels of sdiA in wild type and cured mutant under exogenous AHL and imipenem and when co-cultured with Pseudomonas aeruginosa under imipenem stress. This study highlighted that sub-inhibitory concentration of imipenem in combination with AHL is acting as a signal to SdiA, a quorum-sensing receptor in E. coli.
The transcriptional response of the sdiA gene in E. coli which codes for a quorum-sensing receptor protein is enhanced under imipenem exposure in the presence of acyl homoserine lactone signal molecules.
Publisher
Oxford University Press
Subject
Anti-Bacterial Agents - pharmacology
/ Bacteria
/ E coli
/ Escherichia coli - drug effects
/ Escherichia coli - metabolism
/ Escherichia coli Proteins - genetics
/ Escherichia coli Proteins - metabolism
/ Gene Expression Regulation, Bacterial - drug effects
/ Imipenem
/ Mutants
/ Pseudomonas aeruginosa - drug effects
/ Pseudomonas aeruginosa - genetics
/ Pseudomonas aeruginosa - metabolism
