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A composite critical-size rabbit mandibular defect for evaluation of craniofacial tissue regeneration
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A composite critical-size rabbit mandibular defect for evaluation of craniofacial tissue regeneration
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A composite critical-size rabbit mandibular defect for evaluation of craniofacial tissue regeneration
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Journal Article
A composite critical-size rabbit mandibular defect for evaluation of craniofacial tissue regeneration
2016
Overview
This protocol describes the study design, surgical procedure, postoperative care and analysis techniques necessary to produce a preclinical rabbit model of large bone and tooth defects in the mandible, for use in a wide range of areas in craniofacial tissue engineering.
Translational biomaterials targeted toward the regeneration of large bone defects in the mandible require a preclinical model that accurately recapitulates the regenerative challenges present in humans. Computational modeling and
in vitro
assays do not fully replicate the
in vivo
environment. Consequently,
in vivo
models can have specific applications such as those of the mandibular angle defect, which is used to investigate bone regeneration in a nonload-bearing area, and the inferior border mandibular defect, which is a model for composite bone and nerve regeneration, with both models avoiding involvement of soft tissue or teeth. In this protocol, we describe a reproducible load-bearing critical-size composite tissue defect comprising loss of soft tissue, bone and tooth in the mandible of a rabbit. We have previously used this procedure to investigate bone regeneration, vascularization and infection prevention in response to new biomaterial formulations for craniofacial tissue engineering applications. This surgical approach can be adapted to investigate models such as that of regeneration in the context of osteoporosis or irradiation. The procedure can be performed by researchers with basic surgical skills such as dissection and suturing. The procedure takes 1.5–2 h, with ∼2 h of immediate postoperative care, and animals should be monitored daily for the remainder of the study. For bone tissue engineering applications, tissue collection typically occurs 12 weeks after surgery. In this protocol, we will present the necessary steps to ensure reproducibility; tips to minimize complications during and after surgery; and analytical techniques for assessing soft tissue, bone and vessel regeneration by gross evaluation, microcomputed tomography (microCT) and histology.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
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