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Endothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteins
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Endothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteins
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Journal Article
Endothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteins
2008
Overview
Aim:
Endothelium-dependent relaxations to certain neurohumoral substances are mediated by pertussis toxin-sensitive Gi/o protein. Our experiments were designed to determine the role, if any, of pertussis toxin-sensitive G-proteins in relaxations attributed to endothelium-derived hyperpolarizing factor (EDHF).
Methods:
Pig coronary arterial rings with endothelia were suspended in organ chambers filled with Krebs-Ringer bicarbonate solution maintained at 37 °C and continuously aerated with 95%O
2
and 5% CO
2
. Isometric tension was measured during contractions to prostaglandin F
2α
in the presence of indomethacin and
N
ω
-nitro-
L
-arginine methyl ester (
L
-NAME).
Results:
Thrombin, the thrombin receptor-activating peptide SFLLRN, bradykinin, substance P, and calcimycin produced dose-dependent relaxations. These relaxations were not inhibited by prior incubation with pertussis toxin, but were abolished upon the addition of charybdotoxin plus apamin. Relaxations to the α2-adrenergic agonist UK14304 and those to serotonin were abolished in the presence of indomethacin and
L
-NAME.
Conclusion:
Unlike nitric oxide-mediated relaxations, EDHF-mediated relaxations of pig coronary arteries do not involve pertussis toxin-sensitive pathways and are Gi/o protein independent.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Biological Factors - pharmacology
/ Biomedical and Life Sciences
/ Charybdotoxin - pharmacology
/ Coronary Vessels - drug effects
/ Coronary Vessels - physiology
/ Dose-Response Relationship, Drug
/ Endothelium-Dependent Relaxing Factors - pharmacology
/ Enzyme Inhibitors - pharmacology
/ GTP-Binding Protein alpha Subunits, Gi-Go - metabolism
/ Muscle Relaxation - drug effects
/ Neurotransmitter Agents - pharmacology
/ NG-Nitroarginine Methyl Ester - pharmacology
/ Peptide Fragments - pharmacology
/ Pertussis Toxin - pharmacology
/ Serotonin Antagonists - pharmacology
/ Vaccine
