Asset Details
Do you wish to reserve the book?
Construction and Evaluation of Engineered Yersinia entomophaga for Stable Inheritance of trans-Cry3Aa-T-HasA Against Monochamus alternatus
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Construction and Evaluation of Engineered Yersinia entomophaga for Stable Inheritance of trans-Cry3Aa-T-HasA Against Monochamus alternatus
Do you wish to request the book?
Construction and Evaluation of Engineered Yersinia entomophaga for Stable Inheritance of trans-Cry3Aa-T-HasA Against Monochamus alternatus
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Construction and Evaluation of Engineered Yersinia entomophaga for Stable Inheritance of trans-Cry3Aa-T-HasA Against Monochamus alternatus
2026
Overview
Monochamus alternatus larvae, as concealed trunk-boring pests, evade conventional insecticide contact due to their cryptic feeding niche. To overcome this limitation, previous studies have engineered strains of the naturally entomopathogenic bacterium Yersinia entomophaga. The lethality of these strains against M. alternatus was enhanced by incorporating extracellular secretion systems and enriching insecticidal proteins within the larval midgut. However, plasmid loss occurs during serial subculturing. Here, we established an engineered strain that expresses the red fluorescent protein gene mCherry to explore the applicability of bacterial conjugation transfer to Yersinia. We then constructed a chromosomally integrated strain (CSLH88-pCHSW) that incorporates extracellular secretion systems. The results of stability assays demonstrated 100% retention of the mCherry and Cry3Aa-T-HasA genes over 78 generations. SDS-PAGE and Western blot analyses confirmed the extracellular secretion of the Cry3Aa-T protein in the CSLH88-pCHSW strain. Bioassays revealed that the CSLH88-pCHSW strain was significantly more virulent against M. alternatus larvae than both the wild-type strain (CSLH88) and the plasmid-transformed strain (CSLH88-pCHKW), and exhibited markedly faster insecticidal kinetics. Our study reveals the application of bacterial conjugation transfer technology for constructing biocontrol strains. This genomically stabilized Yersinia strain eliminates the risks of failure associated with plasmid loss in the field, enabling the sustainable control of M. alternatus.
Publisher
MDPI AG,Multidisciplinary Digital Publishing Institute (MDPI)
Subject
/ Bacillus thuringiensis Toxins - genetics
/ Bacteria
/ Bacterial Proteins - genetics
/ Disease
/ Enzymes
/ Genes
/ Genomes
/ Hemolysin Proteins - genetics
/ Hemolysin Proteins - metabolism
/ Luminescent Proteins - genetics
/ Luminescent Proteins - metabolism
/ Pest Control, Biological - methods
/ Proteins
/ Toxicity
/ Toxins
