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A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
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A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
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Journal Article
A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
2020
Overview
In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR–Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as
CXCL9
messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables—via simple visualization—the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
A fast and inexpensive point-of-care assay based on CRISPR–Cas13 accurately detects the DNA of opportunistic viruses in blood and urine samples as well as an mRNA marker of renal transplant rejection in urine samples.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/77
/ 38/90
/ 9/10
/ Assaying
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Blood
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR
/ Cytomegalovirus - isolation & purification
/ Cytomegalovirus Infections - diagnosis
/ DNA
/ Grafting
/ Humans
/ Kidney
/ Kidney Transplantation - adverse effects
/ Male
/ mRNA
/ Opportunistic Infections - diagnosis
/ Pathology, Molecular - methods
/ Polyomavirus - isolation & purification
/ Polyomavirus Infections - diagnosis
/ Postoperative Complications - diagnosis
/ Tumor Virus Infections - diagnosis
/ Urine
