Journal Article

Iron modulates the activity of monoamine oxidase B in SH-SY5Y cells

Hu, Yi, Lu, Huiru, Huang, Hui, Chen, Jun, Chai, Zhifang, Zhou, Mengxue, Zhu, Qing, Yao, Shao Q.

2017

Overview

Both monoamine oxidase B (MAO-B) and iron accumulation are associated with neurologic diseases including Parkinson’s disease. However, the association of iron with MAO-B activity was poorly understood. Here we took advantage of highly sensitive and specific fluorescence probes to examine the change in MAO-B activity in human dopaminergic neuroblastoma (SH-SY5Y) cells upon iron exposure. Both ferric and ferrous ions could significantly enhance the activity of MAO-B, instead of MAO-A, in SH-SY5Y cells. In addition, iron-induced increase in MAO-B probe fluorescence could be prevented by pargyline and other newly developed MAO-B inhibitors, suggesting that it was MAO-B activity-dependent. These findings may suggest MAO-B is an important sensor in iron-stressed neuronal cells.

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: Amine oxidase (flavin-containing) / Biochemistry / Biomedical and Life Sciences / Cell Biology / Cell Line, Tumor / Cellular biology / Chlorides - pharmacology / Dopamine - metabolism / Dopamine receptors / Enzymes / Ferric Compounds - pharmacology / Ferrous Compounds - pharmacology / Ferrous ions / Fluorescence / Fluorescent Dyes - chemistry / Fluorescent indicators / Gene Expression / Humans / Inhibitors / Iron / Life Sciences / Lymphocytes B / Medicine/Public Health / Microbiology / Microscopy, Fluorescence / Monoamine Oxidase - genetics / Monoamine Oxidase - metabolism / Monoamine Oxidase Inhibitors - chemical synthesis / Monoamine Oxidase Inhibitors - pharmacology / Movement disorders / Neurodegenerative diseases / Neurological diseases / Neurons - cytology / Neurons - drug effects / Neurons - enzymology / Neurotoxicity / Oxidase / Pargyline / Pargyline - pharmacology / Parkinson's disease / Pharmacology/Toxicology / Plant Physiology