Asset Details
Do you wish to reserve the book?
Tart Cherry (Prunus cerasus) Extract Exerts High Intracellular ROS Scavenging Activity and Repression of ARE (Antioxidant Response Element) Pathway in Human Hepatocytes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Tart Cherry (Prunus cerasus) Extract Exerts High Intracellular ROS Scavenging Activity and Repression of ARE (Antioxidant Response Element) Pathway in Human Hepatocytes
Do you wish to request the book?
Tart Cherry (Prunus cerasus) Extract Exerts High Intracellular ROS Scavenging Activity and Repression of ARE (Antioxidant Response Element) Pathway in Human Hepatocytes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Tart Cherry (Prunus cerasus) Extract Exerts High Intracellular ROS Scavenging Activity and Repression of ARE (Antioxidant Response Element) Pathway in Human Hepatocytes
2025
Overview
Polyphenol-rich fruits represent promising natural candidates for mitigating oxidative stress. We determined in dose–response manner the intracellular antioxidant activities of P. cerasus (tart cherry) extract in HepG2 cells using three different cellular assays targeting specific mechanisms of action: (1) the AOP1 assay, to assess intracellular ROS scavenging activity; (2) the CAA assay, to estimate ROS scavenging activity at the cell membrane; and (3), the HepG2-ARE-luc assay, to evaluate Antioxidant Response Element (ARE) pathway modulation. Tart cherry extract exhibited a high and concentration-dependent intracellular ROS scavenging activity with the AOP1 assay (EC50 of 72.02 µg/mL), whereas antioxidant efficacy measured via the CAA assay was much lower (EC50 of 6.975 mg/mL). Notably, P. cerasus extract did not activate the ARE-driven luciferase gene expression. Instead, the extract induced a clear dose-dependent repression of ARE-driven transcriptional activity, with a reduction in luciferase gene expression ranging from 20 to 70% across the sample tested concentrations (0.38–98 µg/mL). These findings suggest that, at concentrations where it functions as a potent intracellular ROS scavenger, P. cerasus extract exerts a negative regulation of the ARE pathway. Further investigations are warranted to elucidate the compounds underlying these effects.
Publisher
MDPI AG,Multidisciplinary Digital Publishing Institute (MDPI)
