Journal Article

CD4 T cells in chronic myeloid leukemia present MHC class II-dependent and IFN-γ-dependent cytotoxic capacity

Yao, Zhuoxin, Huang, Yueting, Jiang, Yirong, Li, Zhangkun, Xu, Bing, Yao, Jingwei, Zhou, Yong, Fan, Ziying, Li, Guowei, Li, Zhifeng

2023

Overview

At present, many therapeutic schemes have been used to improve the prognosis of patients with chronic myeloid leukemia (CML), but response remains poor in a small group of patients. CD4 T cell-mediated cytotoxicity has been found in various autoimmune diseases. This study analyzed the characteristics of CD4 T cell mediated cytotoxicity in CML patients and healthy people. The cytotoxicity of CD4 T cells was tested in using two CML cell lines, including the MHC class II-deficient K562 cells and the MHC class II-expressing KU812 cells. CD4 T cell-mediated lysis was minimal in K562 cells but was much higher in KU812 cells. In CML patients, the level of CD4 T cell-mediated lysis was limited to a certain level. Interestingly, pre-treating KU812 cells with IFN-γ could significantly elevate the expression of MHC class II and elevate the level of CD4 T cell-mediated lysis. Overall, these data indicated CD4 T cells could become a potential candidate for cytotoxic elimination of CML cells.

• Publisher: Springer US,Springer Nature B.V
• Subject: Antineoplastic Agents / CD4-Positive T-Lymphocytes / Cytotoxicity / Cytotoxicity, Immunologic / Hematology / Humans / Interferon-gamma / Internal Medicine / Leukemia / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Lymphocytes / Medicine / Medicine & Public Health / Oncology / Original Paper / Pathology