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Epigenetic regulation of LINC01270 in breast cancer progression by mediating LAMA2 promoter methylation and MAPK signaling pathway
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Epigenetic regulation of LINC01270 in breast cancer progression by mediating LAMA2 promoter methylation and MAPK signaling pathway
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Journal Article
Epigenetic regulation of LINC01270 in breast cancer progression by mediating LAMA2 promoter methylation and MAPK signaling pathway
2023
Overview
Application of long non-coding RNAs (lncRNAs) for modulation of breast cancer (BC) has attracted much attention. Here, we probed into the role and underlying mechanism of long intergenic non-coding RNA 01270 (LINC01270) in BC. With the help of bioinformatics tools, we identified laminin subunit alpha 2 (
LAMA2
) as a BC-related differentially expressed gene to discern the effect of LAMA2 in BC cells.
LAMA2
was initially poorly expressed while LINC01270 was highly expressed in BC. BC cells were subsequently treated with sh-LINC01270 or/and sh-LAMA2 for exploration of their regulatory mechanism in BC, which unfolded that LINC01270 inhibition up-regulated
LAMA2
and inactivated the MAPK signaling pathway to suppress malignant characteristics of BC cells. Functional assays demonstrated that LINC01270 bound to
DNMT1
,
DNMT3a
, and
DNMT3b
promoted the methylation of CpG islands in
LAMA2
promoter and inhibited the
LAMA2
expression. Moreover, our data suggested that
LAMA2
suppressed MAPK signaling pathway to inhibit BC cell malignant characteristics. The in vitro results were re-produced with the help of the in vivo experimentations. In conclusion, LINC01270 silencing inhibited the methylation of
LAMA2
promoter to suppress the activation of MAPK signaling pathway, which subsequently restrained the BC progression.
Graphical abstract
1, Overexpression of LAMA2 inhibits malignant features of BC cells.
2, LINC01270 promotes LAMA2 promoter methylation by recruiting DNMTs to the LAMA2 promoter region.
3, 5-aza-dc reverses the promotion of LAMA2 promoter methylation by LINC01270.
4, LAMA2 inhibits malignant features of BC cells by suppressing the activation of MAPK signaling pathway.
Publisher
Springer Netherlands,Springer Nature B.V
