Journal Article

BNST PKCδ neurons are activated by specific aversive conditions to promote anxiety-like behavior

Brown, Jordan A, Simerly, Richard B, Centanni, Samuel W, Calipari, Erin S, Patel, Sachin, Bedenbaugh, Michelle N, Yoon, Hye Jean, Melchior, James R, Sale, Eryn, Negasi, Milen D, Luchsinger, Joseph R, Adank, Danielle N, Winder, Danny G, Taylor, Anne, Williford, Kellie M

2023

Overview

The bed nucleus of the stria terminalis (BNST) is a critical mediator of stress responses and anxiety-like behaviors. Neurons expressing protein kinase C delta (BNSTPKCδ) are an abundant but understudied subpopulation implicated in inhibiting feeding, but which have conflicting reports about their role in anxiety-like behaviors. We have previously shown that expression of PKCδ is dynamically regulated by stress and that BNSTPKCδ cells are recruited during bouts of active stress coping. Here, we first show that in vivo activation of this population is mildly aversive. This aversion was insensitive to prior restraint stress exposure. Further investigation revealed that unlike other BNST subpopulations, BNSTPKCδ cells do not exhibit increased cfos expression following restraint stress. Ex vivo current clamp recordings also indicate they are resistant to firing. To elucidate their afferent control, we next used rabies tracing with whole-brain imaging and channelrhodopsin-assisted circuit mapping, finding that BNSTPKCδ cells receive abundant input from affective, arousal, and sensory regions including the basolateral amygdala (BLA) paraventricular thalamus (PVT) and central amygdala PKCδ-expressing cells (CeAPKCδ). Given these findings, we used in vivo optogenetics and fiber photometry to further examine BNSTPKCδ cells in the context of stress and anxiety-like behavior. We found that BNSTPKCδ cell activity is associated with increased anxiety-like behavior in the elevated plus maze, increases following footshock, and unlike other BNST subpopulations, does not desensitize to repeated stress exposure. Taken together, we propose a model in which BNSTPKCδ cells may serve as threat detectors, integrating exteroceptive and interoceptive information to inform stress coping behaviors.

• Publisher: Nature Publishing Group
• Subject: Amygdala / Anxiety / Arousal / Aversion learning / Behavior / Brain mapping / Emotional behavior / Footshock / Genetics / Information processing / Kinases / Neuroimaging / Optics / Photometry / Protein kinase C / Rabies / Sensory neurons / Stria terminalis / Thalamus