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α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression
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α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression
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Journal Article
α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression
2013
Overview
13
C-α-ketoacid metabolic precursors of phenylalanine and tyrosine effectively enter the metabolism of a protein overexpressing
E. coli
strain to label Phe- and Tyr-residues devoid of any cross-labelling. The methodology gives access to highly selective labelling patterns as valuable tools in protein NMR spectroscopy without the need of
15
N-chiral amino acid synthesis using organic chemistry.
Publisher
Springer Netherlands
