Journal Article

Tenuigenin Prevents IL-1β-induced Inflammation in Human Osteoarthritis Chondrocytes by Suppressing PI3K/AKT/NF-κB Signaling Pathway

Wang, Wenbo, Zeng, Lihong, Liu, Jiakun, Wang, Chunlei, Zhang, Tao

2016

Overview

Tenuigenin (TEN), the main active component of Polygala tenuifolia , has been reported to have anti-inflammatory effects. However, the effects of TEN on IL-1β-stimulated osteoarthritis chondrocytes have not been reported. The purpose of this study was to investigate the anti-inflammatory effects and mechanism of TEN on IL-1β-stimulated human osteoarthritis chondrocytes. Human osteoarthritis chondrocytes were pretreated with or without TEN for 1 h and then stimulated with IL-1β. The production of NO and PGE2 were detected by the Griess reagent and ELISA. The expression of NF-κB and MAPKs (p38, JNK, ERK) were measured by Western blot analysis. The production of MMP-1, MMP3, and MMP13 were measured by ELISA. The results showed that treatment of TEN significantly inhibited IL-1β-induced NO and PGE2 production. TEN also suppressed IL-1β-induced MMP-1, MMP3, and MMP13 expression. Furthermore, TEN was found to inhibit IL-1β-induced NF-κB activation, PI3K, and AKT phosphorylation. In conclusion, these results suggest that TEN inhibits IL-1β-induced inflammation in human osteoarthritis chondrocytes by inhibiting PI3K/AKT/NF-κB signaling pathway.

• Publisher: Springer US
• Subject: Anti-Inflammatory Agents - pharmacology / Arthroplasty, Replacement, Knee / Biomedical and Life Sciences / Biomedicine / Cells, Cultured / Chondrocytes - drug effects / Chondrocytes - pathology / Dinoprostone - biosynthesis / Drugs, Chinese Herbal - pharmacology / Extracellular Signal-Regulated MAP Kinases - biosynthesis / Humans / Immunology / Inflammation - prevention & control / Interleukin-1beta - immunology / Internal Medicine / JNK Mitogen-Activated Protein Kinases - biosynthesis / MAP Kinase Signaling System - drug effects / Matrix Metalloproteinase 1 - biosynthesis / Matrix Metalloproteinase 13 - biosynthesis / Matrix Metalloproteinase 3 - biosynthesis / Nitric Oxide - biosynthesis / Original Article / Osteoarthritis - drug therapy / p38 Mitogen-Activated Protein Kinases - biosynthesis / Pathology / Pharmacology/Toxicology / Phosphatidylinositol 3-Kinases - metabolism / Phosphorylation - drug effects / Polygala / Proto-Oncogene Proteins c-akt - metabolism / Rheumatology / Transcription Factor RelA - metabolism