Journal Article

Endosperm-preferred Expression of Maize Genes as Revealed by Transcriptome-wide Analysis of Expressed Sequence Tags

Nogueira, Fábio T. S., Vettore, André L., e Silva, Thaís Rezende, Fisch, Paulo H., de Rosa, Vincente E., Arruda, Paulo, Rebello, Marcelo M., Neto, Germano Cord, da Silva, Felipe Rodrigues, Verza, Natalia C.

2005

Overview

The transcriptome-wide endosperm-preferred expression of maize genes was addressed by analyzing a large database of expressed sequence tags (ESTs). We generated 30,531 high quality sequence-reads from the 5'-ends of cDNA libraries from maize endosperm harvested at 10, 15, and 20 days after pollination. A further 196,900 maize sequence-reads retrieved from public databases were added to this endosperm collection to generate MAIZEST, a database with tools for data storage and analysis. MAIZEST contains 227,431 ESTs, one third of which represents developing endosperm and the remaining two-thirds represent transcripts from 49 cDNA libraries constructed from different organs and tissues. Assembling the MAIZEST ESTs generated 29,206 putative transcripts, of which a set of 4032 assembled sequences was composed exclusively of sequences derived from endosperm cDNA libraries. After sequence analysis using overlapping parameters, a sub-set of 2403 assembled sequences was functionally annotated and revealed a wide variety of putative new genes involved in endosperm development and metabolism.

• Publisher: Springer Nature B.V
• Subject: cDNA libraries / Corn / Data storage / endosperm / Expressed Sequence Tags / gene banks / gene expression / Gene Expression Profiling / Gene Expression Regulation, Developmental / Gene Expression Regulation, Plant / Gene Expression Regulation, Plant - genetics / genetics / Genomics / growth & development / Libraries / metabolism / Plant Proteins / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Roots / Plant Roots - genetics / Plant Roots - metabolism / Seedlings / Seedlings - genetics / Seedlings - metabolism / Seeds / Seeds - genetics / Transcription, Genetic / Transcription, Genetic - genetics / Zea mays / Zea mays - genetics / Zea mays - growth & development