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Nitric oxide donor regulates Agrobacterium-mediated genetic transformation efficiency in soybean Glycine max (L.) Merrill
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Nitric oxide donor regulates Agrobacterium-mediated genetic transformation efficiency in soybean Glycine max (L.) Merrill
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Journal Article
Nitric oxide donor regulates Agrobacterium-mediated genetic transformation efficiency in soybean Glycine max (L.) Merrill
2020
Overview
The present study investigates the potentiality of Sodium nitroprusside (SNP) to enhance the efficiency of genetic transformation in soybean. Half-seeds cultured on co-cultivation [4.44 μM N6-benzyl adenine (BA) and 30 μM SNP]; shoot induction (4.44 μM BA and 30 μM SNP) and rooting medium [4.93 μM indole 3-butyric acid (IBA) and 30 μM SNP] exhibited improved transformation efficiency (34.6%) in contrast to the regeneration system devoid of SNP (23%). The putatively transformed plants were evaluated by GUS assay and molecular analysis like PCR and Southern hybridization. Furthermore, the transformation system developed herein entails a shorter period (75-days) for developing plantlets from half-seeds of soybean. The outcome of this study revealed that the addition of SNP increased regeneration efficiency of plants, which translated to improved transformation efficiency in soybean.Key messageThis study is the first report employing sodium nitroprusside to enhance transformation efficiency in soybean.
Publisher
Springer Nature B.V
Subject
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