Journal Article

Structural Basis of Reversible Phosphorylation by Maize Pyruvate Orthophosphate Dikinase Regulatory Protein

Tao, Ye, Wang, Bai-chen, Chen, Zhenhang, Wu, Wei, Jiang, Lun, Liu, Yujie, Chen, Zhongzhou, Chen, Yi-bo, Zheng, Jiangge

2016

Overview

Pyruvate orthophosphate dikinase (PPDK) is one of the most important enzymes in C₄ photosynthesis. PPDK regulatory protein (PDRP) regulates the inorganic phosphate-dependent activation and ADP-dependent inactivation of PPDK by reversible phosphorylation. PDRP shares no significant sequence similarity with other protein kinases or phosphatases. To investigate the molecular mechanism by which PDRP carries out its dual and competing activities, we determined the crystal structure of PDRP from maize (Zea mays). PDRP forms a compact homo-dimer in which each protomer contains two separate N-terminal (NTD) and C-terminal (CTD) domains. The CTD includes several key elements for performing both phosphorylation and dephosphorylation activities: the phosphate binding loop (P-loop) for binding the ADP and inorganic phosphate substrates, residues Lys-274 and Lys-299 for neutralizing the negative charge, and residue Asp-277 for protonating and deprotonating the target Thr residue of PPDK to promote nucleophilic attack. Surprisingly, the NTD shares the same protein fold as the CTD and also includes a putative P-loop with AMP bound but lacking enzymatic activities. Structural analysis indicated that this loop may participate in the interaction with and regulation of PPDK. The NTD has conserved intramolecular and intermolecular disulfide bonds for PDRP dimerization. Moreover, PDRP is the first structure of the domain of unknown function 299 enzyme family reported. This study provides a structural basis for understanding the catalytic mechanism of PDRP and offers a foundation for the development of selective activators or inhibitors that may regulate photosynthesis

• Publisher: American Society of Plant Biologists
• Subject: BIOCHEMISTRY AND METABOLISM / Light / Models, Molecular / Mutagenesis, Site-Directed / Phosphates - metabolism / Phosphorylation / Photosynthesis / Plant Proteins - chemistry / Plant Proteins - genetics / Plant Proteins - metabolism / Pyruvate, Orthophosphate Dikinase - chemistry / Pyruvate, Orthophosphate Dikinase - genetics / Pyruvate, Orthophosphate Dikinase - metabolism / Pyruvates - metabolism / Zea mays - enzymology / Zea mays - genetics / Zea mays - physiology