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Fine mechanisms of the interaction of silver nanoparticles with the cells of Salmonella typhimurium and Staphylococcus aureus
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Fine mechanisms of the interaction of silver nanoparticles with the cells of Salmonella typhimurium and Staphylococcus aureus
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Journal Article
Fine mechanisms of the interaction of silver nanoparticles with the cells of Salmonella typhimurium and Staphylococcus aureus
2013
Overview
Silver nanoparticles possess antibacterial effect for various bacteria; however mechanisms of the interaction between Ag-NPs and bacterial cells remain unclear. The aim of our study was to obtain direct evidence of Ag-NPs penetration into cells of Gram-negative bacterium
S. typhimurium
and Gram-positive bacterium
S. aureus
, and to study cell responses to Ag-NPs. The Ag-NPs (most 8–10 nm) were obtained by gas-jet method.
S. typhimurium
(7.81 × 10
7
CFU), or
S. aureus
(8.96 × 10
7
CFU) were treated by Ag-NPs (0.05 mg/l of silver) in orbital shaker at 190 rpm, 37 °C. Bacteria were sampled at 0.5, 1, 1.5, 2, 5 and 23 h of the incubation for transmission electron microscopy of ultrathin sections. The Ag-NPs adsorbed on outer membrane of
S
.
typhimurium
and cell wall of
S. auereus
; penetrated and accumulated in cells without aggregation and damaging of neighboring cytoplasm. In cells of
S. aureus
Ag-NPs bound with DNA fibers. Cell responses to Ag-NPs differed morphologically in
S. typhimurium
and
S. aureus
, and mainly were presented by damage of cell structures. The cytoplasm of
S. aureus
became amorphous, while
S. typhimurium
showed lumping and lysis of cytoplasm which led to formation of “empty” cells. Other difference was fast change of cell shape in
S. typhimurium
, and late deformation of
S. aureus cells
. The obtained results showed how different could be responses induced by the same NPs in relatively simple prokaryotic cells. Evidently, Ag-NPs directly interact with macromolecular structures of living cells and are exert an active influence on their metabolism.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
Anti-Bacterial Agents - chemistry
/ Anti-Bacterial Agents - pharmacology
/ Bacteria
/ Biomedical and Life Sciences
/ Cells
/ Dose-Response Relationship, Drug
/ Fibers
/ Metal Nanoparticles - chemistry
/ Salmonella typhimurium - cytology
/ Salmonella typhimurium - drug effects
/ Silver
/ Staphylococcus aureus - cytology
