Journal Article

TIP60: an actor in acetylation of H3K4 and tumor development in breast cancer

Degoul, Françoise, Besse, Sophie, Bignon, Yves-Jean, Pajon, Amaury, Daures, Marine, Rifaï, Khaldoun, Penault-Llorca, Frédérique, Bernard-Gallon, Dominique, Idrissou, Mouhamed, Judes, Gaëlle, Mishellany, Florence, Dubois, Lucas

2018

Overview

The acetyltransferase TIP60 is reported to be downregulated in several cancers, in particular breast cancer, but the molecular mechanisms resulting from its alteration are still unclear. In breast tumors, H3K4ac enrichment and its link with TIP60 were evaluated by chromatin immunoprecipitation-qPCR and re-chromatin immunoprecipitation techniques. To assess the biological roles of TIP60 in breast cancer, two cell lines of breast cancer, MDA-MB-231 (ER-) and MCF-7 (ER+) were transfected with shRNA specifically targeting TIP60 and injected to athymic Balb-c mice. We identified a potential target of TIP60, H3K4. We show that an underexpression of TIP60 could contribute to a reduction of H3K4 acetylation in breast cancer. An increase in tumor development was noted in sh-TIP60 MDA-MB-231 xenografts and a slowdown of tumor growth in sh-TIP60 MCF-7 xenografts. This is evidence that the underexpression of TIP60 observed in breast cancer can promote the tumorigenesis of ER-negative tumors.

• Publisher: Future Medicine Ltd,Future Medicine (Taylor & Francis)
• Subject: Acetylation / Acetyltransferase / acetyltransferase TIP60 / Binding sites / Brain cancer / Breast cancer / Cancer / Cell cycle / Chromatin / Deoxyribonucleic acid / DNA / DNA methylation / Gene expression / Genomes / H3K4ac / Immunoglobulins / Immunoprecipitation / Life Sciences / Molecular modelling / RNA polymerase / Studies / Target recognition / Tumor cell lines / Tumorigenesis / Tumors / Xenografts / Xenotransplantation