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Discovery and molecular characterization of a novel trichovirus infecting sweet cherry
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Discovery and molecular characterization of a novel trichovirus infecting sweet cherry
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Journal Article
Discovery and molecular characterization of a novel trichovirus infecting sweet cherry
2020
Overview
Contigs with the highest sequence similarity (73%) to Apricot pseudo-chlorotic leaf spot virus (genus Trichovirus, family Betaflexiviridae) were identified by high-throughput sequencing from a symptomless sweet cherry accession. The complete genome sequence of this new virus is 7460 nucleotides, excluding the 3′ poly(A) tail. Its genome organization is very similar to several trichoviruses infecting fruit trees, with three open reading frames encoding putative replicase, movement protein and coat protein (CP). The virus shares amino acid sequence identities of 60–73% at replicase and 53–76% at CP with other trichoviruses. Phylogenetic analyses group it and other trichoviruses in a cluster. These results support that this virus, which is tentatively named cherry latent virus 1, should be considered a new member in the genus Trichovirus.
Publisher
Springer Nature B.V
Subject
