Journal Article

Tart cherry (Prunus cerasus L.) dietary supplement modulates visceral adipose tissue CB1 mRNA levels along with other adipogenesis-related genes in rat models of diet-induced obesity

Mosconi, Gilberto, Moruzzi, Michele, Martinelli, Ilenia, Damiano, Silvia, Palermo, Francesco Alessandro, Tayebati, Seyed Khosrow, Cifani, Carlo, Cocci, Paolo, Tomassoni, Daniele, Lupidi, Giulio, Maggi, Federica, Micioni Di Bonaventura, Maria Vittoria, Amantini, Consuelo

2021

Overview

Purpose There is increasing evidence for the involvement of dietary bioactive compounds in the cross-talk modulation of endocannabinoid system and some of the key regulators of transcriptional control for adipogenesis. Methods We aimed to characterize the expression of cannabinoid CB1/CB2 receptors and fatty acid amide hydrolase (FAAH) along with selected adipogenesis-related genes (PPARγ, SREBP-1c and PREF-1), adipocyte-secreted factors (leptin and adiponectin), mitochondrial bioenergetic modulators (PGC-1A and UCP-2), and transient receptor potential vanilloid subtype 1 (TRPV1) and 2 (TRPV2) channels in visceral adipose tissue of rats fed with a high-fat diet (HFD) containing either tart cherry seeds alone or tart cherry seeds and juice for 17 weeks. The visceral adipose tissue was weighed and checked the expression of different markers by qRT-PCR, Western blot and immunohistochemistry. Results Tart cherry supplements were able to downregulate the HFD-induced mRNA expression of CB1 receptor, SREBP-1c, PPARγ, leptin, TRPV1 and TRPV2 resulting in potential anti-adipogenic effects. Conclusion The present study points out that the intake of bioactive constituents of tart cherry may attenuate the effect of adipogenesis by acting directly on the adipose tissue and modulating the interplay between CB1, PPARγ and TRPV channel gene transcription.

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: Adipogenesis / Adiponectin / Adipose tissue / amide hydrolases / Animal models / Bioactive compounds / Body fat / Cannabinoid CB1 receptors / Cannabinoid CB2 receptors / cannabinoids / Capsaicin receptors / Chemistry / Chemistry and Materials Science / Dietary supplements / Endocannabinoid system / fatty acids / Fatty-acid amide hydrolase / Gene expression / High fat diet / Hydrolase / Immunohistochemistry / Immunomodulation / juices / Leptin / Mitochondria / Nutrition / Obesity / Original Contribution / PGC-1 protein / Prunus cerasus / rats / Seeds / Sterol regulatory element-binding protein / Transcription / transcription (genetics) / Transient receptor potential proteins / transient receptor potential vanilloid channels / Weight control / Western blotting