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Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells
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Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells
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Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells
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Journal Article
Protective effects of bovine milk exosomes against oxidative stress in IEC-6 cells
2021
Overview
Purpose
Bovine milk exosomes, which are enriched with microRNAs (miRNAs) and proteins, regulate immune response and growth. In the present study, we aimed to assess the protective effects of bovine milk exosomes against oxidative stress of intestinal crypt epithelial cells (IEC-6).
Methods
Bovine milk exosomes were isolated and characterized. To assess the protective effects of exosomes, IEC-6 cells were pretreated with exosomes, followed by H
2
O
2
. Cell viability and levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPX), reactive oxidative species (ROS), and lactate dehydrogenase (LDH) were measured. The expression levels of nuclear factor erythroid 2-related factor 2 (
Nrf
2) and heme oxygenase 1 (
Ho1
) genes, and miR-146a, miR-155, and the HO-1 protein were also determined.
Results
The isolated bovine milk exosome were positive for CD63 and CD9 expression. The exosomes were approximately circular and had a diameter of about 67.23 nm. Pretreatment of IEC-6 cells with bovine milk exosomes enhanced cell viability; increased SOD and GPX activities; and reduced LDH, ROS, and MDA levels after H
2
O
2
challenge. Further analysis showed that exosome pretreatment increased intracellular miR-146a and miR-155 levels. Exosome pretreatment inhibited the elevation of
Nrf2
and
Ho1
gene expression induced by H
2
O
2
, but promoted HO-1 protein expression.
Conclusion
The results indicated that bovine milk exosomes exerted protective effects against oxidative stress in IEC-6 cells.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
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