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Metabolic Engineering of Chlamydomonas reinhardtii for Enhanced β-Carotene and Lutein Production
by
Vira Chaitali
, Rathod, Jayant Pralhad
, Prakash Gunjan
, Lali, Arvind M
in
Carotene
/ Chlamydomonas reinhardtii
/ Chlorophyll
/ Gene expression
/ Lutein
/ Lycopene
/ Metabolic engineering
/ Metabolism
/ Photons
/ Phytoene synthase
/ Yeast
/ β-Carotene
2020
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Metabolic Engineering of Chlamydomonas reinhardtii for Enhanced β-Carotene and Lutein Production
by
Vira Chaitali
, Rathod, Jayant Pralhad
, Prakash Gunjan
, Lali, Arvind M
in
Carotene
/ Chlamydomonas reinhardtii
/ Chlorophyll
/ Gene expression
/ Lutein
/ Lycopene
/ Metabolic engineering
/ Metabolism
/ Photons
/ Phytoene synthase
/ Yeast
/ β-Carotene
2020
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While trying to remove the title from your shelf something went wrong :( Kindly try again later!
Do you wish to request the book?
Metabolic Engineering of Chlamydomonas reinhardtii for Enhanced β-Carotene and Lutein Production
by
Vira Chaitali
, Rathod, Jayant Pralhad
, Prakash Gunjan
, Lali, Arvind M
in
Carotene
/ Chlamydomonas reinhardtii
/ Chlorophyll
/ Gene expression
/ Lutein
/ Lycopene
/ Metabolic engineering
/ Metabolism
/ Photons
/ Phytoene synthase
/ Yeast
/ β-Carotene
2020
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Metabolic Engineering of Chlamydomonas reinhardtii for Enhanced β-Carotene and Lutein Production
Journal Article
Metabolic Engineering of Chlamydomonas reinhardtii for Enhanced β-Carotene and Lutein Production
2020
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Overview
The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase – PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synthase (psy) and lycopene cyclization (lcyb) activities. Heterologous expression of this bifunctional PBS gene led to 38% enhancement in β-carotene along with 60% increase in the lutein yields under low light conditions of 75 μmol photons m−2 s−1. Short Duration-High Light induction strategy led to overall 72% and 83% increase in β-carotene and lutein yield reaching up to 22.8 mg g−1 and 8.9 mg g−1, respectively. This is the first report of expression of heterologous bifunctional PBS gene resulting in simultaneous enhancement in β-carotene and lutein content in phototrophic engineered cells.
Publisher
Springer Nature B.V
Subject
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