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Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions
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Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions
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Journal Article
Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions
2022
Overview
In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of A. baumannii belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the A. baumannii CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of Galleria mellonella larvae infected with A. baumannii of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding A. baumannii K types.
Publisher
MDPI AG,MDPI
Subject
Acinetobacter baumannii - chemistry
/ Acinetobacter baumannii - genetics
/ Acinetobacter baumannii - metabolism
/ Bacterial Capsules - genetics
/ Bacterial Capsules - metabolism
/ E coli
/ Enzymes
/ Genomes
/ Glycoside Hydrolases - metabolism
/ Polysaccharides - metabolism
/ Polysaccharides, Bacterial - metabolism
/ Proteins
