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Analysis of Chemical Components and Blood‐Absorbed Components in Youjing Granules by UHPLC‐Q‐Orbitrap‐MS
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Analysis of Chemical Components and Blood‐Absorbed Components in Youjing Granules by UHPLC‐Q‐Orbitrap‐MS
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Journal Article
Analysis of Chemical Components and Blood‐Absorbed Components in Youjing Granules by UHPLC‐Q‐Orbitrap‐MS
2025
Overview
This study investigates the chemical components of Youjing Granules (YG) and identifies blood‐absorbed components in rat serum following YG administration via gavage. Chemical components and blood‐absorbed components of YG were analysed and identified using ultra‐high‐performance liquid chromatography coupled with hybrid quadrupole‐orbitrap high‐resolution mass spectrometry (UHPLC‐Q‐orbitrap‐MS). Identification was achieved by comparing retention time, precise molecular weight, secondary MS fragments with literature data and reference substances. A total of 132 chemical components were identified and analysed from YG, primarily including flavonoids, prenol lipids, organooxygen compounds, isoflavonoids, steroids and steroid derivatives, as well as cinnamic acids and derivatives. Twenty‐four blood‐absorbed components were detected in serum, comprising 15 prototype components and 9 metabolites. The analysis of chemical components and blood‐absorbed components in YG using UPLC‐Q‐orbitrap‐MS technology provides a reference basis for further elucidating the pharmacodynamic material basis and mechanism of action of YG.
Publisher
John Wiley & Sons, Inc,Wiley-VCH
Subject
