Journal Article

Exploring the diversity of promoter and 5′UTR sequences in ancestral, historic and modern wheat

Hammond‐Kosack, Kim E., Hammond‐Kosack, Michael C.U., King, Robert, Kanyuka, Kostya

2021

Overview

A data set of promoter and 5′UTR sequences of homoeo‐alleles of 459 wheat genes that contribute to agriculturally important traits in 95 ancestral and commercial wheat cultivars is presented here. The high‐stringency myBaits technology used made individual capture of homoeo‐allele promoters possible, which is reported here for the first time. Promoters of most genes are remarkably conserved across the 83 hexaploid cultivars used with <7 haplotypes per promoter and 21% being identical to the reference Chinese Spring. InDels and many high‐confidence SNPs are located within predicted plant transcription factor binding sites, potentially changing gene expression. Most haplotypes found in the Watkins landraces and a few haplotypes found in Triticum monococcum, germplasms hitherto not thought to have been used in modern wheat breeding, are already found in many commercial hexaploid wheats. The full data set which is useful for genomic and gene function studies and wheat breeding is available at https://rrescloud.rothamsted.ac.uk/index.php/s/DMCFDu5iAGTl50u/authenticate.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc
• Subject: 5' Untranslated Regions / agronomic traits / Alleles / Binding sites / biotechnology / Chromosomes / Cultivars / data collection / Datasets / Gene expression / Genes / Genomes / Germplasm / Haplotypes / hexaploidy / landraces / Plant breeding / promoter capture / Promoters / repetitive elements (RE) / sequence variation / Single-nucleotide polymorphism / transcription factor binding sites (TFBS) / transcription factors / transposable elements (TE) / Triticum aestivum / Triticum monococcum / Watkins landraces / Wheat