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An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2
An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2
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An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2
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An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2
An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2

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An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2
An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2
Journal Article

An Eco‐Friendly Synthesis and Characterization of Antibacterial, Antifungal, and Antioxidant Silver Nanoparticles From Bioactive Streptomyces sp. Strain WSN‐2

2026
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Overview
The present study reports the isolation and molecular identification of Streptomyces sp. strain WSN‐2 using 16S rRNA gene sequencing and BLASTn analysis (GenBank Accession No. MN128377), followed by its application in the green synthesis of silver nanoparticles (AgNPs). Biomass filtrate of Streptomyces sp. WSN‐2 efficiently reduced silver ions to form stable AgNPs, confirmed by a characteristic UV‐Vis surface plasmon resonance (SPR) peak at 423 nm. Structural and morphological characterization using FTIR, SEM, TEM, and EDX revealed spherical nanoparticles with a smooth texture and well‐dispersed arrangement. TEM analysis indicated particle size predominantly between 50 and 60 nm (overall range 0.83–100 nm), while the zeta potential of –22.9 mV confirmed moderate colloidal stability. EDX spectra displayed strong elemental silver absorption peaks at 3‐4 keV, indicating crystalline Ag formation. The biosynthesized AgNPs exhibited strong antimicrobial activity against wide range of pathogenic microbes. Maximum antibacterial growth inhibition zones were observed against S. typhi (24 ± 1.53 mm), followed by E. coli (23 ± 1.25 mm), B. subtilis (23 ± 1.73 mm), and P. aeruginosa (22 ± 1.53 mm). Antifungal assays revealed highest antifungal activity against A. flavus (16 ± 1.15 mm), and notable inhibition of A. niger (16 ± 1.25 mm), A. fumigatus (15 ± 1.70 mm), and F. oxysporum (14 ± 1.53 mm). MIC values ranged from 8.00 ± 0.05 µg/mL for P. aeruginosa to 18.000.07 µg/mL for A. fumigatus. The AgNPs also demonstrated remarkable antioxidant potential, achieving 65.2% H₂O₂ scavenging activity at 50 µg/mL, surpassing L‐ascorbic acid (45.1%). These findings highlight Streptomyces sp. WSN‐2 as a promising biogenic source for the synthesis of stable AgNPs with significant antibacterial, antifungal, and antioxidant potential. Green‐synthesized AgNPs from Streptomyces sp. WSN‐2 with robust antibacterial, antifungal, and antioxidant properties, revealing a powerful and eco‐friendly route for producing bioactive nanomaterials.