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Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples
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Journal Article
Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples
2020
Overview
Droplet-based microfluidics is a versatile tool to reveal the dose–response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose–response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose–response data for 1D and 2D dose–response screenings are presented.
Publisher
MDPI,MDPI AG
Subject
