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Pancytopenia after simultaneous vaccinations: Dilemma of hematogone versus leukemia
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Journal Article
Pancytopenia after simultaneous vaccinations: Dilemma of hematogone versus leukemia
2023
Overview
Bone marrow examination revealed 30% of blast-like cells, suspecting leukemia (Figure-1). The CD20 was shown as variable expressions from negative to bright consisting of a “J shaped trail pattern” on CD10/CD20 gating (Figure-2). [See PDF for image] Fig. 1 Complete blood counts, bone marrow smear, and flow cytometric analysis in a child with suspected leukemia (a) Changes in platelet counts (b) Changes in hemoglobin and neutrophil counts (c) Blast like cells as immature B lymphoid cells in different stages of hematogones (black arrows) in bone marrow smear (d) Variable expressions of CD10 Phycoerythrin-Cyanin 7 (PC7), CD19 Phycoerythrin-Texas Red-X (ECD), CD33 Phycoerythrin-Cyanin 5 (PC5), myeloperoxidase Phycoerythrin (PE), Cytoplasmic CD3 APC (Allophycocyanin)-Alexa Fluor 750 (A750) and Terminal deoxynucleotidyl transferase Fluorescein Isothiocyanate (FITC) in blastic cells populations using SSC/CD45 Krome Orange (KO) gating [See PDF for image] Fig. 2 Demonstrating hematogones [H] in total B lymphoid cells (a) using SSC/CD19 ECD gating (b) stage 1[H-1] -2[H-2] -3[H-3] hematogones using CD10 A750/ CD19 ECD gating (c) J shaped hematogon using CD A750/CD20 PC5 gating (d) stage 1[H-1] -2[H-2] -3[H-3] hematogones using CD10 A750/ CD45 KO gating (e) stage 1-2-3 hematogones using CD38 APC/ CD45 KO gating (f) stage 1[H-1] -2[H-2] -3[H-3] hematogones using CD10 A750/ CD34 PC7 gating by multiparameter 10-color flow cytometric analysis.
Publisher
Springer India,Springer Nature B.V
Subject
