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eDNA metabarcoding as a means to track distributions of different fish species in a protected area
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eDNA metabarcoding as a means to track distributions of different fish species in a protected area
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eDNA metabarcoding as a means to track distributions of different fish species in a protected area
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Journal Article
eDNA metabarcoding as a means to track distributions of different fish species in a protected area
2024
Overview
In Lar National Park (Caspian Sea basin, Iran), the Caspian trout (Salmo caspius) population faces different threats, including introduced fish species. Due to the harsh environmental conditions and limited accessibility, monitoring of fish species via conventional approaches proves difficult. Hence, environmental DNA metabarcoding may prove an appropriate tool for monitoring fishes within the park. Environmental DNA samples from eight stream sites in the National Park were sequenced via metabarcoding of the 12S rRNA gene, and the species identified via eDNA metabarcoding were compared to the results of electrofishing performed at the same localities on the same day. No significant difference in the number of Caspian Sea trout DNA sequence reads was detected among the collection sites (p > 0.05). The highest number of reads was detected in Dalichay Stream, but the highest population density determined via electrofishing was in Siahpalas Stream. The discrepancy between the eDNA read count and trout population density, as well as the limited sampling scheme within this study, limit our ability to provide a robust conclusion about the application of environmental DNA metabarcoding for assessment of fish density in Lar National Park. Environmental DNA metabarcoding detected more species than electrofishing, but no significant differences in the composition of the local fish community were observed. Introduced fish species were all observed or detected in Siahpalas Stream, which is characterized by high water temperature, muddy substrate, and lower flow rate. A significant effect of flow rate and total dissolved solids on the presence of introduced fish species (p = 0.02) and of flow rate alone on relative abundance of introduced fish species (p = 0.03) was detected. To standardize the application of eDNA metabarcoding as a biodiversity assessment tool in Lar National Park, future studies should characterize the parameters that affect eDNA persistence and detectability in the system.
In Lar National Park (Caspian Sea basin, Iran) Caspian Sea trout populations and non‐native fish diversity and distribution was assessed and compared using eDNA metabarcoding and electrofishing. The results revealed that non‐native fish species were distributed in limited habitats in accordance with distribution of suitable environmental factors, but the Caspian Sea trout existed all over the studied region. Overall, eDNA metabarcoding appeared to better represent fish diversity compared to electrofishing.
