Journal Article

Circular RNA circRHOT1 is upregulated and promotes cell proliferation and invasion in pancreatic cancer

Qu, Shibin, Hao, Xiaokun, Shang, Runze, Song, Wenjie, Wang, Quancheng, Zhang, Xuan, Liu, Zhengcai, Niu, Kunwei, Li, Haimin, Yang, Xisheng

2019

Overview

We aimed to identify the roles of circRHOT1 in pancreatic cancer. The circRHOT1 was acquired from our previous study followed by quantitative real-time PCR and fluorescence hybridization validation in pancreatic cancer. We used siRNA and shRNA to explore the function of circRHOT1 in pancreatic cancer cells. Bioinformatic analyses were applied to study the potential mechanism of circRHOT1. The circRHOT1 was upregulated in pancreatic cancer and predominantly located in the cytoplasm. Reducing the circRHOT1 expression may inhibit the pancreatic cancer cell proliferation, invasion and migration. The circRHOT1 may play a role in pancreatic cancer through binding miR-26b, miR-125a, miR-330 and miR-382 to regulate multiple tumor-associated pathways. This study demonstrated that circRHOT1 may serve as an oncogenic circRNA that promotes tumor progression.

• Publisher: Future Medicine Ltd
• Subject: Binding sites / Cancer / Cell adhesion & migration / Cell culture / Cell cycle / Cell growth / Cell migration / Cell proliferation / ceRNA / circRHOT1 / Circular RNA / Cytoplasm / Fluorescence / Fluorescence in situ hybridization / Identification methods / Lung cancer / Medical prognosis / miRNA / Pancreatic cancer / Ribonucleic acid / RNA / siRNA / Studies / Tumors