Journal Article

Transcriptomic changes and signalling pathways induced by arsenic stress in rice roots

Lin, Chung-Yi, Nguyen, Quynh Thi Thuy, Fu, Shih-Feng, Chen, Ying-Chih, Huang, Tsai-Lien, Huang, Hao-Jen

2012

Overview

Arsenic (As) is considered the most common toxic metalloid, but its molecular mode of action is not well understood. We investigated whether arsenate [As(V)] can induce intracellular reactive oxygen species production and calcium oscillation in rice roots. To better understand the molecular basis of plant cell responses to As, we performed a large-scale analysis of the rice transcriptome during As(V) stress. As(V) induced genes involved in abiotic stress, detoxification pathways and secondary metabolic process. Genes involved in secondary cell wall biogenesis, cell cycle and oligopeptide transport were mainly downregulated. Genes encoding signalling components such as receptor-like cytoplasmic kinases protein kinase, APETALA2/ethylene response factor, heat shock factor, MYB and zinc-finger protein expressed in inflorescence meristem transcription factors were increased in expression. The expression of GARP-G2-like and C3H transcription factors was specifically modulated by As(V) stress. The predominant families of As(V)-regulated transporters belonged to the ATP-binding cassette superfamily and telurite-resistance/dicarboxylate transporters. Several factors involved in signaling, such as mitogen-activated protein kinase (MAPK), MAPK kinase kinase and calcium-dependent protein kinase (CDPK), were also upregulated. Moreover, As(V) markedly increased the activity of MAPKs and CDPK-like kinases, and CDPK and NADPH oxidases were involved in As-induced MAPK activation. Further characterization of these As(V)-responsive genes and signalling pathways may help better understand the mechanisms of metalloid uptake, tolerance and detoxification in plants.

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: Agricultural biotechnology / Arsenates / Arsenates - pharmacokinetics / Arsenates - toxicity / Arsenic / Arsenic content / Biochemistry / Biomedical and Life Sciences / Calcium / Calcium Signaling / Calcium Signaling - drug effects / cell cycle / cell walls / Detoxification / drug effects / Environmental Pollutants / Environmental Pollutants - pharmacokinetics / Environmental Pollutants - toxicity / gene expression regulation / genes / Genes, Plant / Genes, Plant - drug effects / genetics / Inactivation, Metabolic / Inactivation, Metabolic - genetics / Inactivation, Metabolic - physiology / inflorescences / Life Sciences / MAP Kinase Signaling System / MAP Kinase Signaling System - drug effects / mechanism of action / meristems / metabolism / mitogen-activated protein kinase / mitogen-activated protein kinase kinase / Mode of action / Models, Biological / Oryza / Oryza - drug effects / Oryza - genetics / Oryza - metabolism / Oryza sativa / pharmacokinetics / physiology / Phytochelatins / Phytochelatins - metabolism / Plant biology / Plant Growth Regulators / Plant Growth Regulators - genetics / Plant Growth Regulators - metabolism / Plant Pathology / Plant Proteins / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Roots / Plant Roots - drug effects / Plant Roots - genetics / Plant Roots - metabolism / Plant Sciences / plant stress / reactive oxygen species / Reactive Oxygen Species - metabolism / Rice / Roots / Signal Transduction / Signal Transduction - drug effects / Stress response / Stress, Physiological / toxicity / transcription (genetics) / transcription factors / Transcription Factors - genetics / Transcription Factors - metabolism / transcriptome / Transcriptome - drug effects / transporters