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Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120
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Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120
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Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120
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Journal Article
Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120
2016
Overview
The hypothetical protein ‘Alr3200’ of
Anabaena
sp. strain PCC7120 is highly conserved among cyanobacterial species. It is a member of the DUF820 (Domain of Unknown Function) protein family, and is predicted to have a DNase domain. Biochemical analysis revealed a Mg(II)-dependent DNase activity for Alr3200 with a specific activity of 8.62×10
4
Kunitz Units (KU) mg
−1
protein. Circular dichroism analysis predicted Alr3200 to have ~40% β-strands and ~9% α-helical structures.
Anabaena
PCC7120 inherently expressed Alr3200 at very low levels, and its overexpression had no significant effect on growth of
Anabaena
under control conditions. However, An
alr3200
+
, the recombinant
Anabaena
strain overexpressing Alr3200, exhibited zero survival upon exposure to 6 kGy of γ-radiation, which is the LD
50
for wild type
Anabaena
PCC7120 as well as the vector control recombinant strain, AnpAM. Comparative analysis of the two recombinant
Anabaena
strains suggested that it is not the accumulated Alr3200
per se
, but its possible interactions with the radiation-induced unidentified DNA repair proteins of
Anabaena
, which hampers DNA repair resulting in radiosensitivity.
Publisher
Springer India,Springer Nature B.V
Subject
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Biomedical and Life Sciences
/ circular dichroism spectroscopy
/ Deoxyribonucleases - biosynthesis
/ Deoxyribonucleases - chemistry
/ DNA
/ Gene Expression Regulation, Bacterial
/ Protein Conformation, alpha-Helical
/ Protein Conformation, beta-Strand
/ Proteins
/ Zoology
