Journal Article

Effects of dietary n-6:n-3 PUFA ratio on fatty acid composition, free amino acid profile and gene expression of transporters in finishing pigs

Li, Yinghui, Tang, Yulong, Li, Fengna, Duan, Yehui, Geng, Meimei, Oladele, Oso Abimbola, Kim, Sung Woo, Yin, Yulong

2015

Overview

Revealing the expression patterns of fatty acid and amino acid transporters as affected by dietary n-6:n-3 PUFA ratio would be useful for further clarifying the importance of the balance between n-6 and n-3 PUFA. A total of ninety-six finishing pigs were fed one of four diets with the ratio of 1:1, 2·5:1, 5:1 and 10:1. Pigs fed the dietary n-6:n-3 PUFA ratio of 5:1 had the highest (P< 0·05) daily weight gain, and those fed the dietary n-6:n-3 PUFA ratio of 1:1 had the largest loin muscle area (P< 0·01). The concentration of n-3 PUFA was raised as the ratio declined (P< 0·05) in the longissimus dorsi and subcutaneous adipose tissue. The contents of tryptophan, tasty amino acids and branched-chain amino acids in the longissimus dorsi were enhanced in pigs fed the dietary n-6:n-3 PUFA ratios of 1:1–5:1. The mRNA expression level of the fatty acid transporter fatty acid transport protein-1 (FATP-1) was declined (P< 0·05) in the longissimus dorsi of pigs fed the dietary n-6:n-3 PUFA ratios of 1:1–5:1, and increased (P< 0·05) in the subcutaneous adipose tissue of pigs fed the dietary n-6:n-3 PUFA ratios of 5:1 and 10:1. The expression profile of FATP-4 was similar to those of FATP-1 in the adipose tissue. The mRNA expression level of the amino acid transceptors LAT1 and SNAT2 was up-regulated (P< 0·05) in the longissimus dorsi of pigs fed the dietary n-6:n-3 PUFA ratios of 1:1 and 2·5:1. In conclusion, maintaining the dietary n-6:n-3 PUFA ratios of 1:1–5:1 would facilitate the absorption and utilisation of fatty acids and free amino acids, and result in improved muscle and adipose composition.

• Publisher: Cambridge University Press
• Subject: absorption / Adipose tissue / Amino Acid Transport System A - genetics / Amino Acid Transport System A - metabolism / amino acid transporters / Amino acids / Amino Acids - analysis / Amino Acids - metabolism / Animals / Crosses, Genetic / Diet / Diet - veterinary / Dietary Fats - analysis / Energy Intake / fatty acid composition / Fatty Acid Transport Proteins - genetics / Fatty Acid Transport Proteins - metabolism / Fatty acids / Fatty Acids - analysis / Fatty Acids - metabolism / Fatty Acids, Omega-3 - administration & dosage / Fatty Acids, Omega-3 - analysis / Fatty Acids, Omega-3 - metabolism / Fatty Acids, Omega-6 - administration & dosage / Fatty Acids, Omega-6 - analysis / Fatty Acids, Omega-6 - metabolism / finishing / free amino acids / free fatty acids / Gene expression / Gene Expression Regulation, Developmental / Hogs / Large Neutral Amino Acid-Transporter 1 - genetics / Large Neutral Amino Acid-Transporter 1 - metabolism / Linseed Oil - administration & dosage / Linseed Oil - metabolism / longissimus muscle / Meat - analysis / messenger RNA / Metabolism / Metabolism and Metabolic Studies / Muscle Development / Muscle, Skeletal - growth & development / Muscle, Skeletal - metabolism / omega-3 fatty acids / polyunsaturated fatty acids / Protein Isoforms - genetics / Protein Isoforms - metabolism / Soybean Oil - administration & dosage / Soybean Oil - metabolism / Subcutaneous Fat - growth & development / Subcutaneous Fat - metabolism / Sus scrofa - growth & development / Sus scrofa - metabolism / Swine / swine feeding / tryptophan / Weight Gain