Asset Details
Do you wish to reserve the book?
An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana
Do you wish to request the book?
An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana
2019
Overview
Heterodimeric complexes containing the lipase-like protein ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) are regarded as central regulators of plant innate immunity. In this context, a complex of EDS1 with PHYTOALEXIN DEFICIENT4 (PAD4) is required for basal resistance and signaling downstream of immune receptors containing an N-terminal Toll-interleukin-1 receptor-like domain (TNLs) in Arabidopsis (Arabidopsis thaliana). Here we analyze EDS1 functions in the model Solanaceous plant Nicotiana benthamiana (Nb). Stable Nb mutants deficient in EDS1 complexes are not impaired in basal resistance, a finding which contradicts a general role for EDS1 in immunity. In Nb, PAD4 demonstrated no detectable immune functions, but TNL-mediated resistance responses required EDS1 complexes incorporating a SENESCENCE ASSOCIATED GENE101 (SAG101) isoform. Intriguingly, SAG101 is restricted to those genomes also encoding TNL receptors, and we propose it may be required for TNL-mediated immune signaling in most plants, except the Brassicaceae. Transient complementation in Nb was used for accelerated mutational analyses while avoiding complex biotic interactions. We identify a large surface essential for EDS1-SAG101 immune functions that extends from the N-terminal lipase domains to the C-terminal EDS1-PAD4 domains and might mediate interaction partner recruitment. Furthermore, this work demonstrates the value of genetic resources in Nb, which will facilitate elucidation of EDS1 functions.
Publisher
American Society of Plant Biologists (ASPB),American Society of Plant Biologists
Subject
/ Arabidopsis Proteins - chemistry
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Bacterial Infections - immunology
/ Carboxylic Ester Hydrolases - genetics
/ Carboxylic Ester Hydrolases - metabolism
/ DNA-Binding Proteins - chemistry
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Plant Diseases - microbiology
/ Plants, Genetically Modified
/ Receptors, Cell Surface - genetics
/ Receptors, Cell Surface - metabolism
/ Signal Transduction - genetics
/ Signal Transduction - immunology
/ Solanum lycopersicum - genetics
