Asset Details
Do you wish to reserve the book?
ALKBH5 Promotes the Proliferation of Glioma Cells via Enhancing the mRNA Stability of G6PD
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
ALKBH5 Promotes the Proliferation of Glioma Cells via Enhancing the mRNA Stability of G6PD
Do you wish to request the book?
ALKBH5 Promotes the Proliferation of Glioma Cells via Enhancing the mRNA Stability of G6PD
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
ALKBH5 Promotes the Proliferation of Glioma Cells via Enhancing the mRNA Stability of G6PD
2021
Overview
This study aims to investigate the biological role of 6-methyladenine (m6A) methylation in inducing the carcinogenesis of glioma and its proliferation. Relative levels of ALKBH5 and glucose-6-phosphate dehydrogenase (G6PD) in glioma tissues and cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Gain-of-function and loss-of-function approaches were used to investigate the role of ALKBH5 in mediating proliferation and energy metabolism of glioma cells. The regulatory effect of ALKBH5 on G6PD was analyzed using m6A-qRT-PCR. Our results showed that ALKBH5 was upregulated in glioma, which stimulated glioma cells to proliferate. Serving as a m6A eraser, ALKBH5 demethylated the target transcript G6PD and enhanced its mRNA stability, thereby promoting G6PD translation and activating the pentose phosphate pathway (PPP). Collectively, ALKBH5 stimulates glioma cells to proliferate through erasing the m6A methylation of G6PD, which can be utilized as a potential therapeutic target for glioma.
Publisher
Springer US,Springer Nature B.V
Subject
AlkB Homolog 5, RNA Demethylase - biosynthesis
/ AlkB Homolog 5, RNA Demethylase - genetics
/ Biomedical and Life Sciences
/ Brain Neoplasms - metabolism
/ Cell Proliferation - physiology
/ Enzymes
/ Glioma
/ Glucose
/ Glucose 6 phosphate dehydrogenase
/ Glucosephosphate dehydrogenase
/ Glucosephosphate Dehydrogenase - genetics
/ Glucosephosphate Dehydrogenase - metabolism
/ Humans
/ Pentose
/ Plasmids
