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A rapid one-step multiplex RT-PCR assay for the simultaneous detection of five citrus viroids in China
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A rapid one-step multiplex RT-PCR assay for the simultaneous detection of five citrus viroids in China
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Journal Article
A rapid one-step multiplex RT-PCR assay for the simultaneous detection of five citrus viroids in China
2009
Overview
Citrus plants are natural hosts of five viroid species and large numbers of sequence variants. In this paper a simple and sensitive one step multiplex RT-PCR protocol with an internal control was utilised to simultaneously detect and differentiate five citrus viroids:
Citrus exocortis viroid
(CEVd),
Citrus bent leaf viroid
(CBLVd),
Hop stunt viroid
(HSVd),
Citrus viroid-III
(CVd-III) and
Citrus viroid-IV
(CVd-IV). In addition, a micro and rapid total nucleic acid extraction method was developed and the protocol applied to evaluate the occurrence and distribution of citrus viroids in China.
Publisher
Springer Netherlands,Springer,Springer Nature B.V
