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Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus
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Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus
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Journal Article
Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus
2017
Overview
Increasing the self-resistance levels of Streptomyces is an effective strategy to improve the production of antibiotics.To increase the oxytetracycline（OTC） production in Streptomyces rimosus,we investigated the cooperative effect of three co-overexpressing OTC resistance genes：one gene encodes a ribosomal protection protein（otrA） and the other two express efflux proteins（otrB and otrC）.Results indicated that combinational overexpression of otrA,otrB,and otrC（MKABC） exerted a synergetic effect.OTC production increased by 179%in the recombinant strain compared with that of the wild-type strain M4018.The resistance level to OTC was increased by approximately two-fold relative to the parental strain,thereby indicating that applying the cooperative effect of self-resistance genes is useful to improve OTC production.Furthermore,the previously identified cluster-situated activator OtcR was overexpressed in MKABC in constructing the recombinant strain MKRABC;such strain can produce OTC of approximately7.49 g L~（（-1））,which represents an increase of 19%in comparison with that of the OtcR-overexpressing strain alone.Our work showed that the cooperative overexpression of self-resistance genes is a promising strategy to enhance the antibiotics production in Streptomyces.
Publisher
Science China Press,Springer Nature B.V
Subject
/ Bacterial Proteins - genetics
/ Biomedical and Life Sciences
/ Biosynthetic Pathways - genetics
/ Drug Resistance, Bacterial - genetics
/ Gene Expression Regulation, Bacterial - genetics
/ Industrial Microbiology - methods
/ Membrane Transport Proteins - genetics
/ Oxytetracycline - biosynthesis
/ Self
/ Streptomyces rimosus - genetics
/ Streptomyces rimosus - metabolism
/ 合作
/ 土霉素
/ 抗性基因
/ 抗生素生产
/ 过度表达
/ 重组菌株
/ 龟裂链霉菌
