Journal Article

Comparison of the Bioactive Properties of Human and Bovine Hemoglobin Hydrolysates Obtained by Enzymatic Hydrolysis: Antimicrobial and Antioxidant Potential of the Active Peptide α137-141

Diab, Mira Abou, BioEcoAgro - UMR transfrontalière INRAe - UMRT1158 ; Université d'Artois (UA)-Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA) ; Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Deracinois, Barbara, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc) ; Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Gressier, Bernard, Eto, Bruno, Dhaouefi, Jihen, Université de Lille, Flahaut, Christophe, Nedjar, Naïma, Outman, Ahlam, Institut Charles Viollette (ICV) - ULR 7394 (ICV) ; Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille-Institut National d

2023

Overview

This study focuses on the enzymatic hydrolysis of hemoglobin, the main component of cruor that gives blood its red color in mammals. The antibacterial and antioxidant potentials of human hemoglobin hydrolysates were evaluated in comparison to bovine hemoglobin. The results showed strong antimicrobial activity of the peptide hydrolysates against six bacterial strains, independent of the initial substrate concentration level. The hydrolysates also showed strong antioxidant activity, as measured by four different tests. In addition, the antimicrobial and antioxidant activities of the human and bovine hemoglobin hydrolysates showed little or no significant difference, with only the concentration level being the determining factor in their activity. The results of the mass spectrometry study showed the presence of a number of bioactive peptides, the majority of which have characteristics similar to those mentioned in the literature. New bioactive peptides were also identified in human hemoglobin, such as the antibacterial peptides PTTKTYFPHF (α37-46), FPTTKTYFPH (α36-45), TSKYR (α137-141), and STVLTSKYR (α133-141), as well as the antioxidant TSKYR (α137-141). According to these findings, human hemoglobin represents a promising source of bioactive peptides beneficial to the food or pharmaceutical industries.

• Publisher: MDPI,CCSD,MDPI AG
• Subject: Antibacterial agents / Antimicrobial agents / Antioxidants / Blood donors / Comparative analysis / E coli / Enzymes / Glycosylated hemoglobin / Hemoglobin / Hydrolysis / Life Sciences / Lipids / Oxidation / Peptides / Proteins / R&D / Research & development / Salmonella
• ISBN: 0010606219000