Asset Details
Do you wish to reserve the book?
In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin
Do you wish to request the book?
In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin
2018
Overview
Helicobacter pylori
is an infectious agent commonly associated with gastrointestinal diseases. The use of probiotics to treat this infection has been documented, however, their potential antimicrobial metabolites have not yet been investigated. In the present study, the effect of reuterin produced by
Lactobacillus reuteri
on
H. pylori
growth and virulence gene expression was evaluated. It was observed that reuterin caused significant (
P
< 0.05)
H. pylori
growth inhibition at concentrations from 0.08 to 20.48 mM, with minimal inhibitory concentrations (MICs) of 20.48 mM for
H. pylori
ATCC700824 and 10.24 mM for
H. pylori
ATCC43504. In a reuterin bacterial killing assay, it was observed that half of the MIC value for
H. pylori
(ATCC700824) significantly (
P
< 0.01) reduced colony numbers from 5.65 ± 0.35 to 3.78 ± 0.35 Log
10
CFU/mL after 12 h of treatment and then increased them to 5.25 ± 0.23 Log
10
CFU/mL at 24 h; at its MIC value (20.48 mM), reuterin abrogated (
P
< 0.01)
H. pylori
(ATCC700824) growth after 20 h of culture. In addition, reuterin significantly (
P
< 0.01) reduced
H. pylori
(ATCC 43504) colony numbers from 5.65 ± 0.35 to 4.1 ± 0.12 Log10 CFU/mL from 12 to 24 h of treatment and abrogated its growth at its MIC value (10.24 mM), after 20 h of treatment. Reuterin did not alter normal human gastric Hs738.St/Int cell viability at the concentrations tested for
H. pylori
strains. Furthermore, 10 μM reuterin was shown to significantly (
P
< 0.01) reduce mRNA relative expression levels of
H. pylori
virulence genes
vacA
and
flaA
at 3 h post-treatment, whose effect was higher at 6 h post-treatment, as measured by RT-qPCR. The observed direct antimicrobial effect and the downregulation of expression of virulence genes on
H. pylori
by reuterin may contribute to the understanding of the mechanisms of action of probiotics against
H. pylori
.
Publisher
Springer US
Subject
Anti-Bacterial Agents - metabolism
/ Anti-Bacterial Agents - pharmacology
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Chemistry and Materials Science
/ genes
/ Glyceraldehyde - analogs & derivatives
/ Glyceraldehyde - pharmacology
/ Helicobacter Infections - microbiology
/ Helicobacter pylori - drug effects
/ Helicobacter pylori - genetics
/ Helicobacter pylori - growth & development
/ Helicobacter pylori - metabolism
/ Humans
/ Lactobacillus reuteri - metabolism
/ minimum inhibitory concentration
/ quantitative polymerase chain reaction
/ reverse transcriptase polymerase chain reaction
