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Primate Lentiviral Virion Infectivity Factors Are Substrate Receptors That Assemble with Cullin 5-E3 Ligase through a HCCH Motif to Suppress APOBEC3G
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Primate Lentiviral Virion Infectivity Factors Are Substrate Receptors That Assemble with Cullin 5-E3 Ligase through a HCCH Motif to Suppress APOBEC3G
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Journal Article
Primate Lentiviral Virion Infectivity Factors Are Substrate Receptors That Assemble with Cullin 5-E3 Ligase through a HCCH Motif to Suppress APOBEC3G
2005
Overview
Cullin-Ring E3 ubiquitin ligases target substrates for ubiquitin-dependent, proteasome-mediated degradation and regulate critical cellular processes. These cullins assemble with cellular substrate receptor proteins through specific adaptor molecules. F-box- and BC-box-containing receptors use Skp1, ElonginB, and ElonginC as adaptors to recruit Cul1/Cul7 and Cul2/Cul5, respectively. At present, the determinants of Cul2 vs. Cul5 specificity for the BC-box-containing receptors are poorly defined. Here, we demonstrate that primate lentiviral Vif (virion infectivity factor) proteins represent previously uncharacterized substrate receptor proteins that contain divergent BC-box motifs. These molecules selectively assemble with a Cul5-E3 ligase to suppress the antiviral activity of autologous cytidine deaminase APOBEC3G. A previously unrecognized Hx5 Cx17-18 Cx3-5 H motif that is highly conserved among all primate lentiviral Vif proteins was found to be critical for the selective assembly and activity of Vif-Cul5-E3 ligase. Non-primate lentiviral Vif proteins, which lack this HCCH motif, displayed reduced interaction with Cul5. These data suggest that in addition to target protein specificity, substrate receptor proteins play important roles in cullin selection and functional assembly of cullin-Ring E3 ligases. The discovery of these viral substrate receptor molecules that recruit Cul5 through distinct mechanisms from cellular proteins may facilitate the identification of additional cellular factors that regulate cellular functions through Cul5-E3 ligase. Motifs in Vif that are absent from cellular proteins could also be targets for the development of innovative therapeutics.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Cullin Proteins - metabolism
/ Elongin
/ Enzymes
/ Gene Products, vif - genetics
/ Gene Products, vif - metabolism
/ HIV 1
/ Humans
/ Lentiviruses, Primate - metabolism
/ Nucleoside Deaminases - metabolism
/ Primates
/ Proteins
/ Repressor Proteins - metabolism
/ Transcription Factors - metabolism
/ Ubiquitin-Protein Ligase Complexes - metabolism
/ Virions
/ Viruses
