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Induction of MCP-1 Expression in Airway Epithelial Cells: Role of CCR2 Receptor in Airway Epithelial Injury
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Induction of MCP-1 Expression in Airway Epithelial Cells: Role of CCR2 Receptor in Airway Epithelial Injury
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Induction of MCP-1 Expression in Airway Epithelial Cells: Role of CCR2 Receptor in Airway Epithelial Injury
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Journal Article
Induction of MCP-1 Expression in Airway Epithelial Cells: Role of CCR2 Receptor in Airway Epithelial Injury
2002
Overview
The repair of an injured bronchial epithelial cell (BEC) monolayer requires proliferation and migration of BECs into the injured area. We hypothesized that BEC monolayer injury results in monocyte chemoattractant protein-1 (MCP-1) production, which initiates the repair process. BECs (BEAS-2B from ATCC) were utilized in this study. MCP-1 interacts with CCR2B receptor (CCR2B), resulting in cell proliferation, haptotaxis, and healing of the monolayer. Reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to verify the presence of CCR2B. CCR2B was not merely present but also inducible by interleukin-2 (IL-2) and lipopolysaccharide (LPS). We demonstrated by immunohistochemistry that BECs express MCP-1 after injury and that receptor expression can be regulated by exposure to IL-2 and LPS. Haptotactic migration of cells was enhanced in the presence of MCP-1 and reduced in the presence of CCR2B antibody. This enhanced or depressed ability of the BECs to perform haptotactic migration was shown to be statistically significant (P < 0.05) when compared to controls. Finally, BECs proliferate in response to MCP-1 as proven by electric cell-substrate impedance sensing (ECIS) technology. MCP-1-specific antibodies were shown to neutralize the MCP-1-mediated BEC proliferation. This cascade of events following injury to the bronchial epithelium may provide insight into the mechanism of the repair process.
Publisher
Kluwer/Plenum,Springer Nature B.V
Subject
Biological and medical sciences
/ Chemokine CCL2 - biosynthesis
/ Epithelial Cells - metabolism
/ Epithelial Cells - pathology
/ Fundamental and applied biological sciences. Psychology
/ Humans
/ Interleukin-2 - pharmacology
/ Lipopolysaccharides - pharmacology
/ Molecular and cellular biology
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