Journal Article

Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary-Phase Autolysis Resulting in Toxin Release in Clostridium difficile

Butel, Marie-José, Hamiot, Audrey, El Meouche, Imane, Lai-Kuen, René, Fenaille, François, Courtin, Pascal, Pons, Jean-Louis, Chapot-Chartier, Marie-Pierre, Peltier, Johann, Wydau-Dematteis, Sandra, Saubaméa, Bruno, Dupuy, Bruno

2018

Overview

Clostridium difficile is the major etiologic agent of antibiotic-associated intestinal disease. Pathogenesis of C. difficile is mainly attributed to the production and secretion of toxins A and B. Unlike most clostridial toxins, toxins A and B have no signal peptide, and they are therefore secreted by unusual mechanisms involving the holin-like TcdE protein and/or autolysis. In this study, we characterized the cell surface protein Cwp19, a newly identified peptidoglycan-degrading enzyme containing a novel catalytic domain. We purified a recombinant His 6 -tagged Cwp19 protein and showed that it has lytic transglycosylase activity. Moreover, we observed that Cwp19 is involved in cell autolysis and that a C. difficile cwp19 mutant exhibited delayed autolysis in stationary phase compared to the wild type when bacteria were grown in brain heart infusion (BHI) medium. Wild-type cell autolysis is correlated to strong alterations of cell wall thickness and integrity and to release of cytoplasmic material. Furthermore, we demonstrated that toxins were released into the extracellular medium as a result of Cwp19-induced autolysis when cells were grown in BHI medium. In contrast, Cwp19 did not induce autolysis or toxin release when cells were grown in tryptone-yeast extract (TY) medium. These data provide evidence for the first time that TcdE and bacteriolysis are coexisting mechanisms for toxin release, with their relative contributions in vitro depending on growth conditions. Thus, Cwp19 is an important surface protein involved in autolysis of vegetative cells of C. difficile that mediates the release of the toxins from the cell cytosol in response to specific environment conditions. IMPORTANCE Clostridium difficile -associated disease is mainly known as a health care-associated infection. It represents the most problematic hospital-acquired infection in North America and Europe and exerts significant economic pressure on health care systems. Virulent strains of C. difficile generally produce two toxins that have been identified as the major virulence factors. The mechanism for release of these toxins from bacterial cells is not yet fully understood but is thought to be partly mediated by bacteriolysis. Here we identify a novel peptidoglycan hydrolase in C. difficile , Cwp19, exhibiting lytic transglycosylase activity. We show that Cwp19 contributes to C. difficile cell autolysis in the stationary phase and, consequently, to toxin release, most probably as a response to environmental conditions such as nutritional signals. These data highlight that Cwp19 constitutes a promising target for the development of new preventive and curative strategies. Clostridium difficile -associated disease is mainly known as a health care-associated infection. It represents the most problematic hospital-acquired infection in North America and Europe and exerts significant economic pressure on health care systems. Virulent strains of C. difficile generally produce two toxins that have been identified as the major virulence factors. The mechanism for release of these toxins from bacterial cells is not yet fully understood but is thought to be partly mediated by bacteriolysis. Here we identify a novel peptidoglycan hydrolase in C. difficile , Cwp19, exhibiting lytic transglycosylase activity. We show that Cwp19 contributes to C. difficile cell autolysis in the stationary phase and, consequently, to toxin release, most probably as a response to environmental conditions such as nutritional signals. These data highlight that Cwp19 constitutes a promising target for the development of new preventive and curative strategies.

• Publisher: American Society for Microbiology
• Subject: Autolysis / Bacteria / Bacterial Proteins / Bacterial Proteins - chemistry / Bacterial Proteins - genetics / Bacterial Proteins - metabolism / Bacteriolysis / Catalytic Domain / Cell surface / Cell Wall / Cell Wall - genetics / Cell Wall - metabolism / Cell walls / Chemical bonds / Clostridium difficile / Clostridium difficile - enzymology / Clostridium difficile - genetics / Clostridium difficile - growth & development / Clostridium difficile - physiology / Clostridium Infections / Clostridium Infections - microbiology / Cytosol / Environmental conditions / Enzymes / Gene Expression Regulation, Bacterial / Genomes / Gram-positive bacteria / Growth conditions / Health care / Holins / Humans / Life Sciences / Microbiology and Parasitology / Molecular weight / Nosocomial infection / Peptides / Peptidoglycan Glycosyltransferase / Peptidoglycan Glycosyltransferase - chemistry / Peptidoglycan Glycosyltransferase - genetics / Peptidoglycan Glycosyltransferase - metabolism / Peptidoglycan hydrolase / Physiology / Proteins / Stationary phase / Streptococcus infections / Toxins / Vegetative cells / Virulence / Virulence factors