Journal Article

Osilodrostat Is a Potential Novel Steroidogenesis Inhibitor for the Treatment of Cushing Syndrome: An In Vitro Study

van Koetsveld, Peter M, Hofland, Leo J, de Jong, Frank H, Franssen, Gaston J H, Feelders, Richard A, de Rijke, Yolanda B, Creemers, Sara G

2019

Overview

Abstract Context Metyrapone and ketoconazole, frequently used steroidogenesis inhibitors for treatment of Cushing syndrome, can be associated with side effects and limited efficacy. Osilodrostat is a CYP11B1 and CYP11B2 inhibitor, with unknown effects on other steroidogenic enzymes. Objective To compare the effects of osilodrostat, metyrapone, and ketoconazole on adrenal steroidogenesis, and pituitary adenoma cells in vitro. Methods HAC15 cells, 17 primary human adrenocortical cell cultures, and pituitary adenoma cells were incubated with osilodrostat, metyrapone, or ketoconazole (0.01 to 10 µM). Cortisol and ACTH were measured using chemiluminescence immunoassays, and steroid profiles by liquid chromatography-mass spectrometry. Results In HAC15 cells, osilodrostat inhibited cortisol production more potently (IC50: 0.035 µM) than metyrapone (0.068 µM; P < 0.0001), and ketoconazole (0.621 µM; P < 0.0001). IC50 values of osilodrostat and metyrapone for basal cortisol production varied with a 25- and 18-fold difference, respectively, with comparable potency. Aldosterone production was inhibited more potently by osilodrostat vs metyrapone and ketoconazole. Osilodrostat and metyrapone treatment resulted in strong inhibition of corticosterone and cortisol, 11-deoxycortisol accumulation, and modest effects on adrenal androgens. No pituitary-directed effects of osilodrostat were observed. Conclusions Under our study conditions, osilodrostat is a potent cortisol production inhibitor in human adrenocortical cells, comparable with metyrapone. All steroidogenesis inhibitors showed large variability in sensitivity between primary adrenocortical cultures. Osilodrostat might inhibit CYP11B1 and CYP11B2, in some conditions to a lesser extent CYP17A1 activity, and a proximal step in the steroidogenesis. Osilodrostat is a promising treatment option for Cushing syndrome, and in vivo differences with metyrapone are potentially driven by pharmacokinetic differences. The in vitro effects of osilodrostat on human adrenal steroidogenesis are studied compared with metyrapone and ketoconazole. We show that it is a promising novel treatment of Cushing syndrome.

• Publisher: Endocrine Society,Oxford University Press
• Subject: ACTH / Adenoma / Adrenal glands / Adrenocorticotropic hormone / Aldosterone / Aldosterone - biosynthesis / Aldosterone synthase / Androgens / Cabergoline / Cell Culture Techniques / Chemiluminescence / Corticosterone / Cortodoxone - metabolism / Cushing syndrome / Cushing Syndrome - drug therapy / Cytochrome P-450 / Cytochrome P-450 CYP11B2 - antagonists & inhibitors / Drug therapy / Enzyme Inhibitors - pharmacokinetics / Ethylenediaminetetraacetic acid / Hormones / Humans / Hydrocortisone - biosynthesis / Hydroxyprogesterone / Imidazoles - pharmacokinetics / Ketoconazole / Ketoconazole - pharmacokinetics / Liquid chromatography / Mass spectrometry / Mass spectroscopy / Medical research / Medicine, Experimental / Metyrapone / Metyrapone - pharmacokinetics / Nervous system diseases / Pharmacokinetics / Pituitary / Pyridines - pharmacokinetics / Steroid 11-beta-Hydroxylase - antagonists & inhibitors / Steroid 11β-hydroxylase / Steroidogenesis / Tumors