Journal Article

Niche-based screening identifies small-molecule inhibitors of leukemia stem cells

Carpenter, Anne E, Huang, Christina, Logan, David J, Steensma, David P, Hartwell, Kimberly A, Tothova, Zuzana, Golub, Todd R, Hasaka, Thomas, Narayan, Rajiv, Moore, Malcolm A S, Schreiber, Stuart L, Dai, Mingji, Chattopadhyay, Shrikanta, Shamji, Alykhan F, Haydu, J Erika, Kindler, Thomas, Stewart, Alison L, Negri, Joseph M, Kahn, Alissa R, Shterental, Sebastian, Scadden, David T, Tolliday, Nicola J, Bittker, Joshua A, Munoz, Benito, Duvet, Mildred, Miller, Peter G, Mukherjee, Siddhartha, Ebert, Benjamin L, Clemons, Paul A, Järås, Marcus, Al-Shahrour, Fatima, Stern, Andrew M, Shieh, Jae Hung, Dancik, Vlado, Puram, Rishi, Chu, Lisa P, Gilliland, D Gary

2013

Overview

High-throughput screening systems that better mimic the physiological complexity of diseased tissues may aid the discovery of more efficacious compounds. A co-culture system that mimics the microenvironment of leukemia stem cells (LSCs) in bone marrow enables the discovery of compounds, including lovastatin, that selectively kill LSCs. Efforts to develop more effective therapies for acute leukemia may benefit from high-throughput screening systems that reflect the complex physiology of the disease, including leukemia stem cells (LSCs) and supportive interactions with the bone marrow microenvironment. The therapeutic targeting of LSCs is challenging because LSCs are highly similar to normal hematopoietic stem and progenitor cells (HSPCs) and are protected by stromal cells in vivo . We screened 14,718 compounds in a leukemia-stroma co-culture system for inhibition of cobblestone formation, a cellular behavior associated with stem-cell function. Among those compounds that inhibited malignant cells but spared HSPCs was the cholesterol-lowering drug lovastatin. Lovastatin showed anti-LSC activity in vitro and in an in vivo bone marrow transplantation model. Mechanistic studies demonstrated that the effect was on target, via inhibition of HMG-CoA reductase. These results illustrate the power of merging physiologically relevant models with high-throughput screening.

• Publisher: Nature Publishing Group US,Nature Publishing Group
• Subject: 13/106 / 13/89 / 14 / 14/35 / 14/63 / 49/47 / 631/154/1435/2417 / 631/532 / 631/67/1059 / 631/92/507 / 64 / Antineoplastic Agents - pharmacology / Biochemical Engineering / Biochemistry / Bioorganic Chemistry / Bone marrow / Cell Biology / Cell Line, Tumor / Chemistry / Chemistry/Food Science / Cholesterol / Drug Screening Assays, Antitumor - methods / Hematopoietic Stem Cells / Humans / Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology / Leukemia / Lovastatin - pharmacology / Molecules / Neoplastic Stem Cells - cytology / Neoplastic Stem Cells - drug effects / Neoplastic Stem Cells - physiology / Physiology / Stem cells