Journal Article

The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis

Rolfe, D. J., Martin-Fernandez, M. L., Hawes, C., Webb, S. E. D., Runions, J., McKenna, J. F., Tolmie, A. F., Botchway, S. W.

2019

Overview

SignificanceThe plant plasma membrane acts as the front line for cellular perception of the environment. As such, signaling and transport proteins which perceive or transport environmental signals, developmental cues, and nutrients are located within it. A number of studies have revealed that proteins located within the plasma membrane do not simply freely diffuse within its plane. Rather, proteins are localized in nanodomains. In addition to the plasma membrane, plant cells also have an extracellular matrix, the cell wall. Here we have shown that the cell wall has a role in regulating the dynamics and size of plasma-membrane nanodomains for proteins involved in morphogenesis (PIN3) and pathogen perception (FLS2). Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport, and cellular signaling. For these proteins to function effectively there needs to be structure within the PM allowing, for example, proteins in the same signaling cascade to be spatially organized. Here we demonstrate that several proteins with divergent functions are located in clusters of differing size in the membrane using subdiffraction-limited Airyscan confocal microscopy. Single particle tracking reveals that these proteins move at different rates within the membrane. Actin and microtubule cytoskeletons appear to significantly regulate the mobility of one of these proteins (the pathogen receptor FLS2) and we further demonstrate that the cell wall is critical for the regulation of cluster size by quantifying single particle dynamics of proteins with key roles in morphogenesis (PIN3) and pathogen perception (FLS2). We propose a model in which the cell wall and cytoskeleton are pivotal for regulation of protein cluster size and dynamics, thereby contributing to the formation and functionality of membrane nanodomains.

• Publisher: National Academy of Sciences
• Subject: Actin / Actin Cytoskeleton - metabolism / Actin Cytoskeleton - ultrastructure / Arabidopsis / Biological Sciences / Cell Biology / Cell Wall - metabolism / Cell Wall - ultrastructure / Cell walls / Clusters / Confocal microscopy / Cytoskeleton / Membrane Microdomains - metabolism / Membrane Microdomains - ultrastructure / Membranes / Microtubules - metabolism / Microtubules - ultrastructure / Morphogenesis / Particle tracking / Pathogens / Proteins / Signaling / Single Molecule Imaging / Solute transport