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Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
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Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
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Journal Article
Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
2021
Overview
Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource and its size is limited. Here, we investigated how the highly versatile 'knallgas' bacterium Cupriavidus necator reallocates protein resources when grown on different limiting substrates and with different growth rates. We determined protein quantity by mass spectrometry and estimated enzyme utilization by resource balance analysis modeling. We found that C. necator invests a large fraction of its proteome in functions that are hardly utilized. Of the enzymes that are utilized, many are present in excess abundance. One prominent example is the strong expression of CBB cycle genes such as Rubisco during growth on fructose. Modeling and mutant competition experiments suggest that CO 2 -reassimilation through Rubisco does not provide a fitness benefit for heterotrophic growth, but is rather an investment in readiness for autotrophy.
Publisher
eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
Subject
/ Bacterial Proteins - biosynthesis
/ Biomass
/ Carbon
/ Computational and Systems Biology
/ Cupriavidus necator - enzymology
/ Cupriavidus necator - growth & development
/ Cupriavidus necator - metabolism
/ Enzymes
/ Genomes
/ Other
/ Peptides
/ Proteins
/ Ribulose-bisphosphate carboxylase
/ Ribulose-Bisphosphate Carboxylase - genetics
