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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
by
Harrison, Melissa M
, Wildonger, Jill
, Nguyen, Jennifer N
, Gratz, Scott J
, Hamm, Danielle C
, O’Connor-Giles, Kate M
, Cummings, Alexander M
, Donohue, Laura K
in
Animals
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR-Cas Systems
/ Drosophila
/ Drosophila - genetics
/ Drosophila Proteins - genetics
/ Endodeoxyribonucleases - genetics
/ Endodeoxyribonucleases - metabolism
/ Engineering
/ Gene Targeting
/ Genetic Engineering - methods
/ Genome, Insect
/ Genomes
/ Germ-Line Mutation
/ Homologous Recombination
/ Ribonucleic acid
/ RNA
2013
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
by
Harrison, Melissa M
, Wildonger, Jill
, Nguyen, Jennifer N
, Gratz, Scott J
, Hamm, Danielle C
, O’Connor-Giles, Kate M
, Cummings, Alexander M
, Donohue, Laura K
in
Animals
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR-Cas Systems
/ Drosophila
/ Drosophila - genetics
/ Drosophila Proteins - genetics
/ Endodeoxyribonucleases - genetics
/ Endodeoxyribonucleases - metabolism
/ Engineering
/ Gene Targeting
/ Genetic Engineering - methods
/ Genome, Insect
/ Genomes
/ Germ-Line Mutation
/ Homologous Recombination
/ Ribonucleic acid
/ RNA
2013
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Do you wish to request the book?
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
by
Harrison, Melissa M
, Wildonger, Jill
, Nguyen, Jennifer N
, Gratz, Scott J
, Hamm, Danielle C
, O’Connor-Giles, Kate M
, Cummings, Alexander M
, Donohue, Laura K
in
Animals
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR-Cas Systems
/ Drosophila
/ Drosophila - genetics
/ Drosophila Proteins - genetics
/ Endodeoxyribonucleases - genetics
/ Endodeoxyribonucleases - metabolism
/ Engineering
/ Gene Targeting
/ Genetic Engineering - methods
/ Genome, Insect
/ Genomes
/ Germ-Line Mutation
/ Homologous Recombination
/ Ribonucleic acid
/ RNA
2013
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
Journal Article
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
2013
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Overview
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
Publisher
Genetics Society of America
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