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A single step duplex PCR to distinguish Entamoeba histolytica from Entamoeba dispar
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A single step duplex PCR to distinguish Entamoeba histolytica from Entamoeba dispar
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A single step duplex PCR to distinguish Entamoeba histolytica from Entamoeba dispar
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Journal Article
A single step duplex PCR to distinguish Entamoeba histolytica from Entamoeba dispar
2004
Overview
In this study, a single-step duplex polymerase chain reaction procedure was developed for rapid, specific and sensitive identification of Entamoeba histolytica and for its diagnostic differentiation from E. dispar. Specific oligonucleotide primers were combined for the amplification of a cysteine proteinase 5 gene target sequence of 242 bp, present only in E. histolytica. Additionally, another oligonucleotide primer pair for both the E. histolytica and E. dispar actin gene target of 300 bp was designed to amplify only from amoebae DNA. The PCR developed was specific and efficiently identified and differentiated these parasites from each other in either cultured parasites or from stool material.
Publisher
Cambridge University Press
Subject
/ Animals
/ Bacteria
/ Biological and medical sciences
/ Cysteine Endopeptidases - chemistry
/ Cysteine Endopeptidases - genetics
/ Dysentery, Amebic - parasitology
/ Entamoeba histolytica - classification
/ Entamoeba histolytica - enzymology
/ Entamoeba histolytica - genetics
/ Entamoeba histolytica–Entamoeba dispar diagnosis
/ Fundamental and applied biological sciences. Psychology
/ General aspects and techniques. Study of several systematic groups. Models
/ Humans
/ PCR
/ Polymerase Chain Reaction - methods
/ Proteins
